prevalence of extended-spectrum and metallo β-lactamase production in ampc β-lactamase producing pseudomonas aeruginosa isolates from burns

Authors

roya rafiee department of microbiology, faculty of biological sciences, shahid beheshti university, tehran, ir iran

fereshteh eftekhar department of microbiology, faculty of biological sciences, shahid beheshti university, tehran, ir iran; department of microbiology, faculty of biological sciences, shahid beheshti university, tehran, ir iran. tel: +98-2129903208, fax: +98-2122431664

seyyed ahmad tabatabaei department of pediatrics, shahid beheshti university of medical sciences, tehran, ir iran

dariush minaee tehrani department of microbiology, faculty of biological sciences, shahid beheshti university, tehran, ir iran

abstract

conclusions a high prevalence of multiple β-lactamase production was observed among the ampc producers (60%), of which the majority co-produced ampc and mbl. the current study results showed correlation between β-lactamase production and the presence of antibiotic resistance genes in the isolates. results all isolates were multidrug resistant. ampc, esbl and mbl production were observed in 35 (68.6%), 20 (39.2%) and 19 (37.3%) isolates, respectively. overall, 43 isolates (84.3%) carried β-lactamase genes, out of which 31 (60.8%) harbored blaampc, 20 (39.2%) had blatem and 11 (21.6%) carried blaper-1 genes. among the ampc producers, two isolates (6.5%) carried blaampc + blaesbl, 13 (41.9%) had blaampc + blambl and six (19.4%) produced the three enzymes. objectives the current study aimed to determine the prevalence of class a esbl and mbl production in relation to the presence of their related genes among ampc β-lactamase producing p. aeruginosa isolated from burns. materials and methods the antimicrobial susceptibility of 51 p. aeruginosa isolates from patients with burns was examined against 13 antibiotics by the disc diffusion method. minimum inhibitory concentrations (mic) for imipenem and ceftazidime were measured by the microdilution method. ampc production was detected by ampc disc and the modified three-dimensional extract tests. esbl phenotype was confirmed by the double disc synergy test (ddst). presence of β-lactamase genes was detected by specific primers and polymerase chain reaction (pcr). background pseudomonas aeruginosa is one of the most common causes of nosocomial infections. resistance of p. aeruginosa to β-lactam antibiotics may be the result of acquired resistance through mutation and over production of various antibiotic inactivating enzymes. this research aimed to determine the prevalence of extended-spectrum β-lactamases (esbl) and metallo β-lactamase (mbl) production as well as the presence of their related genes among ampc β-lactamase producing p. aeruginosa isolated from burns.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Prevalence of Extended-Spectrum and Metallo β-Lactamase Production in AmpC β-Lactamase Producing Pseudomonas aeruginosa Isolates From Burns

BACKGROUND Pseudomonas aeruginosa is one of the most common causes of nosocomial infections. Resistance of P. aeruginosa to β-lactam antibiotics may be the result of acquired resistance through mutation and over production of various antibiotic inactivating enzymes. This research aimed to determine the prevalence of extended-spectrum β-lactamases (ESBL) and metallo β-lactamase (MBL) production ...

full text

coexistence of ampc and extended-spectrum β-lactamases in metallo-β-lactamase producing pseudomonas aeruginosa burn isolates in tehran

conclusions this study showed that multiple β-lactamases can be produced in burn isolates. this suggests that use of cephalosporins and carbapenems should be restricted in burn isolates to minimize the development and spread of these multidrug resistant pathogens. results there was 99% resistance to carbenicillin, and ticarcillin, 98% to cotrimoxazole, 96% to ciprofloxacin, and aztreonam, 95% t...

full text

Prevalence of AmpC β -Lactamase-Producing Pseudomonas aeruginosa Isolates From Feacal Matter of Cow

Environmental microorganisms represent the most relevant reservoir of resistance to antibiotics in the community, and this is due in part to the undue exposure of these organisms to antimicrobial agents. AmpC β-lactamases are clinically important cephalosporinases encoded on the chromosomes of bacteria in the Enterobacteriaceae family and some non-enteric bacteria including Pseudomonas aerugino...

full text

Epidemiology and Characteristics of Metallo-β-Lactamase-Producing Pseudomonas aeruginosa

Metallo-β-lactamase-producing Pseudomonas aeruginosa (MPPA) is an important nosocomial pathogen that shows resistance to all β-lactam antibiotics except monobactams. There are various types of metallo-β-lactamases (MBLs) in carbapenem-resistant P. aeruginosa including Imipenemase (IMP), Verona integron-encoded metallo-β-lactamase (VIM), Sao Paulo metallo-β-lactamase (SPM), Germany imipenemase (...

full text

Characteristics of Metallo-β-Lactamase-Producing Pseudomonas aeruginosa in Korea

BACKGROUND The aim of this study was to investigate the molecular epidemiological characteristics of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa clinical isolates in Korea. MATERIALS AND METHODS Three hundred and twenty nine P. aeruginosa clinical isolates were collected from 23 general hospitals in Korea from March to June 2014. Species were identified by matrix-assited laser ...

full text

Drug resistance, AmpC-β-lactamase and extended-spectrum β-lactamase-producing Enterobacteriaceae isolated from fish and shrimp

The present study aims to detect the production of extended-spectrum beta-lactamases (ESBL) by enterobacteria isolated from samples of fresh shrimp and fish obtained from the retail trade of the city of Sobral, Ceará State, Brazil. All bacterial isolates were submitted to identification and antimicrobial susceptibility testing using aminopenicillin, beta-lactamase inhibitors, carbapenem, 1st, 2...

full text

My Resources

Save resource for easier access later


Journal title:
jundishapur journal of microbiology

جلد ۷، شماره ۹، صفحات ۰-۰

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023